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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1 (D265A), kappa
- 形态:
Liquid
- 保存条件:
-20°C
- 克隆性:
单克隆
- 保质期:
12 months
- 级别:
一抗
- 供应商:
武汉益普生物科技有限公司
- 宿主:
Mouse
- 应用范围:
FCM, Isotype Control, In vivo Control
- 抗体英文名:
Mouse IgG1 (D265A), kappa Isotype Control antibody (SAA0336)
- 规格:
100μg/1mg
| 规格: | 100μg | 产品价格: | ¥1560.0 |
|---|---|---|---|
| 规格: | 1mg | 产品价格: | ¥6240.0 |
| 产品名 | Mouse IgG1 (D265A), kappa Isotype Control antibody (SAA0336) |
| 货号 | FMK30210 |
| 靶标 | IgG1 (D265A),kappa |
| 克隆号 | SAA0336 |
| 克隆类型 | Monoclonal |
| 同种型 | IgG1 (D265A), kappa |
| 宿主 | Mouse |
| 应用 | FCM, Isotype Control, In vivo Control |
| 纯度 | >95% as determined by SDS-PAGE. |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4, 0.09% Sodium azide. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 说明 | For research use only. |

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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
, isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1 Isotype-Dependent Properties of Monoclonal
和相同的免疫球蛋白及亚型的免疫球蛋白,用于消除由于抗体非特异性与细胞结合而产生的背景染色。同型对照是真正意思上的阴性对照,它不但可以用来设定流式细胞仪的电压,而且还可以帮忙省去昂贵与繁琐的重组细胞因子竞争封闭步骤。在流式细胞仪上样前,染色方式如下: 样本管:一抗+样本 同型对照管:同型对照+样本 2 、如何选择同型对照? 样本管:纯化的CDw25+PE标记的抗Mouse IgG1
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
me why this works!) before cloning out for variants. b) You can screen by ELISA or with mAb coupled RBC using mouse monoclonal antibodies against the Y3 derived Kappa 1a (RG11/15.5) and the specific DA Kappa 1b (G9/1.3), or the appropriate heavy chain (eg NORIG
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