- ￥1700 - 4000
- GeneTex
- 美国
- GTX638567
- 2025年07月14日
- ELISA
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full length human TNF alpha recombinant protein.
- 亚型：
IgG, Kappa
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX638567
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
ELISA
- 浓度：
Batch dependent(Please refer to the vial label for the specific concentration.)
- 靶点：
TNF alpha
- 抗体英文名：
TNF alpha antibody [HL2361]
- 抗体名：
TNF alpha 抗体 [HL2361]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Sandwich ELISA detection of recombinant 293T expressed, human Fc-tagged TNF alpha (54-233 a.a.) using antibodies as below.
Capture: TNF alpha antibody [HL2363] (GTX638569) (5 μg/mL)
Detection: HRP-conjugated TNF alpha antibody [HL2361] (GTX638567) (1 μg/mL)
Please notice that GTX638567 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638569. Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA detection of recombinant 293T expressed, human Fc-tagged TNF alpha (54-233 a.a.) using antibodies as below.
Capture: TNF alpha antibody [HL2361] (GTX638567) (5 μg/mL)
Detection: HRP-conjugated TNF alpha antibody [HL2363] (GTX638569) (1 μg/mL)
Please notice that GTX638569 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638567. Please contact us for custom HRP-conjugated antibody.
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文献和实验[3H] Choline Labeling and TNF Treatment of HL-60 Cells
). Always keep track of rest time!!! --> Equilibrate for longer periods to prevent controls from fluctuating. 10) Treat cells with 30 nM TNF in BSA for appropriate period.
【求助】有什么办法可以阻滞LPS刺激的TNF-alpha分泌？
sonny_zan 我实验的关键步骤，现在就是不知道有什么办法可以阻滞LPS刺激后PBMC分泌TNF-alpha，考虑过用p38 MAPK抑制剂SB203580，后来查到一篇文献说SB203580对LPS刺激后p38 MAPK的活性影响甚微或无。 请各位大侠给点意见啦！我快急死了~~~ xiongran 拿抗体把PBMC表面的TLR4封闭掉试试看！ pureclear 可以考虑
Choline Labeling and TNF Treatment of HL-60 C
Choline Labeling and TNF Treatment of HL-60 Cells Contributor: Suprya Jayadev Date: Aug. 6, 1991 1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum. 2) Pellet cells and wash
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