- ￥1700 - 4000
- GeneTex
- 美国
- GTX635572
- 2025年07月04日
- WB, ICC/IF
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Chk1 (phosPhPo Ser345). The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX635572
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Chk1 (phospho Ser345)
- 抗体英文名：
Chk1 (phospho Ser345) antibody [HL122]
- 抗体名：
Chk1 (phospho Ser345) 抗体 [HL122]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phosphpo Ser345) antibody [HL122] (GTX635572) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Chk1 (phosphpo Ser345) antibody [HL122] detects Chk1 (phosphpo Ser345) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HCT116 cells were fixed in 4% PFA at RT for 15 min.
Green: Chk1 (phosphpo Ser345) stained by Chk1 (phosphpo Ser345) antibody [HL122] (GTX635572) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phosphpo Ser345) antibody [HL122] (GTX635572) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Methods for Studying Checkpoint Kinases Chk1
-terminal regulatory domain. Within the regulatory domain there are two residues, Serine-317 (S317) and Serine-345 (S345), which are phosphorylated in active Chk1 molecules, and subsequently dephosphorylated to inactivate Chk1 and allow mitotic entry
Mammalian CHK1 is a Ser/Thr effector kinase that plays critical roles in the DNA damage-activated cell cycle checkpoint signaling pathway downstream of ATR (ATM and Rad3-related protein kinase). This chapter is focused on describing an assay
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
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