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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the center region of Cathepsin D Light chain. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX56271
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Cathepsin D Light chain
- 抗体英文名:
Cathepsin D Light chain antibody
- 抗体名:
Cathepsin D Light chain 抗体
- 规格:
100 μl
WB analysis of colchicine-treated HepG2 (A), mouse heart (B), rat heart (C) whole cell lysates using GTX56271 Cathepsin D Light chain antibody.
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文献和实验Enzyme Analysis: Cathepsin D as an Example
in high concentration) by direct light absorbency, or by catalytic activity. For example, the protease enzyme, savinase, which is used as an ingredient in washing powder, was determined directly by its absorbency at 200 nm (1 ). For catalytic activity
Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
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