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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant full-size PCNA (S. cerevisiae, 1-258 aa)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Saccharomyces cerevisiae
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX64144
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IP, ChIP assay
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
PCNA (S. cerevisiae)
- 抗体英文名:
PCNA (S. cerevisiae) antibody
- 抗体名:
PCNA (S. cerevisiae) 抗体
- 规格:
100 μg
Western Blot of PCNA in crude extract of S. cerevisiae The antibody was used at 1/1,000 dilution. Molecular mass of PCNA is 29 kDa. Crude extract of S. cerevisiae strain BY4741 (20 μg) applied.
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文献和实验Liao CC et al., EMBO Rep 2020 (PMID:32484313)
Detection of PCNA Modifications in Saccharomyces cerevisiae
PCNA modifications by members of the ubiquitin family are associated with a range of different transactions during replication of damaged and undamaged DNA. This chapter describes detailed protocols for the detection and isolation
Expression of VHH Antibody Fragments in Saccharomyces cerevisiae
The use of Saccharomyces cerevisiae as a production host for heterologous proteins has several advantages compared to the use of a bacterial host. First, S. cerevisiae has proven to be a productive host capable of expressing many heterologous
In Vitro PCNA Modification Assays
proteins from E. coli or S. cerevisiae as well as protocols for setting up small-scale ubiquitylation and sumoylation reactions with budding yeast PCNA. In addition, we provide a method for the purification of monoubiquitylated PCNA for further biochemical
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