- ￥5000
- GeneTex
- 美国
- GTX55778
- 2025年07月12日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant fusion protein containing a sequence corresponding to amino acids 27-175 of human REG3A (NP_002571.1).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX55778
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
REG III alpha
- 抗体英文名：
REG III alpha antibody
- 抗体名：
REG III alpha 抗体
- 规格：
100 μl
IHC-P analysis of rat lung tissue using GTX55778 REG III alpha antibody.
Dilution : 1:100
IHC-P analysis of mouse stomach tissue using GTX55778 REG III alpha antibody.
Dilution : 1:100
IHC-P analysis of mouse lung tissue using GTX55778 REG III alpha antibody.
Dilution : 1:100
WB analysis of various sample lysates using GTX55778 REG III alpha antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane
ICC/IF analysis of MCF7 cells using GTX55778 REG III alpha antibody.
Blue : DAPI
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
% ），这使得化学合成siRNA 的成本升高。体外转录制备长片断RNA 并不难，但是由于研究表明长片断的双链RNA （>29bps ）在哺乳动物细胞中通常会引发抗病毒反应―― 一种非特异基因表达抑制，而目前体外转录往往无法制备小于29bp 的小分子siRNA s 。一个新的研究发现，用RNase III 降解长片断双链RNA 成为siRNA s 库也能够有效诱导特异的基因沉默，这样可以避免了筛选有效siRNA s 的漫长过程，从而快速，经济的实现特定基因表达的沉默。在线虫、果蝇和其他一些物种的RNA 干扰
Polymerase III in vitro Transcription
: 4.0 μl 5X Pol III transcription buffer 0.2 μl 0.1 M DTT 0.2 μl alpha amanatin (1 mg/ml) 0.2 μl RNAse Inhibitor H2O to a final volume of 16 microliters less the maximum volume of protein to be added. Aliquot the second mix to all reaction tubes
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