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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human Exonuclease 1. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX109891
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF
- 浓度:
0.72 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Exonuclease 1
- 抗体英文名:
Exonuclease 1 antibody
- 抗体名:
Exonuclease 1 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Exonuclease 1 antibody detects Exonuclease 1 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% PFA at RT for 15 min.
Green: Exonuclease 1 protein stained by Exonuclease 1 antibody (GTX109891) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Immunofluorescence analysis of PFA-fixed HeLa, using Exonuclease 1(GTX109891) antibody at 1:500 dilution.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Exonuclease 1 antibody (GTX109891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (50 μg of whole cell lysate)
A: mouse thymus
7.5% SDS PAGE
GTX109891 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Truong LN et al., Proc Natl Acad Sci U S A 2013 (PMID:23610439)
Chen YJ et al., Sci Rep 2017 (PMID:28630472)
Hampp S et al., Proc Natl Acad Sci U S A 2016 (PMID:27407148)
Zhou Y et al., Nucleic Acids Res 2014 (PMID:24362840)
Zhou Y et al., J Biol Chem 2013 (PMID:24220101)
Xiao H et al., Cells 2022 (PMID:35805183)
Elb?k CR et al., Cell Rep 2022 (PMID:35045293)
Rall-Scharpf M et al., Aging (Albany NY) 2021 (PMID:34506302)
Kim JJ et al., Mol Cell 2020 (PMID:32966758)
Volcic M et al., Nat Microbiol 2020 (PMID:32690953)
Burdova K et al., EMBO J 2019 (PMID:31424118)
Site-Directed Mutagenesis of Antibody-Variable Regions
Introduction of mutations in antibody combining sites provides a powerful means to assess the functional role of individual residues and segments of the combining sites in interactions with antigen. Once cloned antibody fragments
the ChIP?exo methodology, which combines chromatin immunoprecipitation (ChIP) with lambda exonuclease digestion followed by high?throughput sequencing. ChIP?exo allows identification of a nearly complete set of the binding locations of DNA?binding proteins
of the lon protease. Reduces degradation of b-galactosidase fusion proteins to enhance antibody screening of l libraries. malA Inability to utilize maltose. proAB Mutants require proline for growth in minimal media. recA Gene central to general
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