- ￥4300
- GeneTex
- 美国
- GTX70017
- 2025年07月13日
- WB, ICC/IF, IP, ELISA
- Rabbit
- Human, Mouse, Rat, Kangaroo rat
企业认证
相关产品推荐更多 >
![GSK3 beta antibody [3D10]](https://img1.dxycdn.com/2023/0711/451/6106825198874858761-14.jpg!wh200)
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding phospho Ser55 of human Hec1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Kangaroo rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX70017
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IP, ELISA
- 浓度：
0.26 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Hec1 (phospho Ser 55)
- 抗体英文名：
Hec1 (phospho Ser 55) antibody
- 抗体名：
Hec1 (phospho Ser 55) 抗体
- 规格：
100 μl
Immunofluorescent staining of Hec1 phospho Ser55 in cells treated with Hec1 siRNA (bottom) or control siRNA (top)
GFP-tagged Hec1 wild type, S55A or 6A (Ser55 and five other Ser/Thr residues changed to Ala) mutant was ectopically expressed in HeLa cells and the cell lysate was used for WB analysis with the Hec1 pSer55 antibody (diluted at 1:500)
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 (phospho Ser 55) antibody (GTX70017) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Purified 6his-Hec1 was subject to a kinase assay with recombinant Aurora B and gamma-p32-labelled ATP. The resultant phospho-Hec1 were detected by measuring radioactive signal and by WB analysis with pSer55 antibody
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Sobajima T et al., J Cell Biol 2023 (PMID:36897279)
Kenji Iemura et al., J Cell Biol 2021 (PMID:33988677)
Lu Yang et al., Mol Biol Cell 2021 (PMID:33909454)
Gao L et al., Front Cell Dev Biol 2020 (PMID:32733898)
Asai Y et al., Sci Rep 2020 (PMID:32973131)
Smith RJ et al., Cell Rep 2019 (PMID:31433993)
Asai Y et al., J Cell Biol 2019 (PMID:31527146)
Taveras C et al., Cell Cycle 2019 (PMID:31122175)
Richard J Smith et al., bioRxiv 2019 (Epub)
Nguyen AL et al., Curr Biol 2018 (PMID:30415701)
Vallot A et al., Curr Biol 2018 (PMID:29276128)
Qian J et al., Mol Cell 2017 (PMID:29129638)
Saurin AT et al., Methods Mol Biol 2016 (PMID:27193859)
Hafner J et al., Nat Commun 2014 (PMID:25048371)
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
技术资料暂无技术资料 索取技术资料
