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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The clone AT66G9 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EPHA4 protein.
- 亚型:
IgG2b
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX57687
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Based on internal testing result, this antibody recognizes the extracellular domain of Human EPHA2.
- 抗体英文名:
EphA2 antibody [AT66G9]
- 抗体名:
EphA2 抗体 [AT66G9]
- 规格:
100 μl
WB analysis of various samples using GTX57687 EphA2 antibody.
Lane 1 : Recombinant Human EPHA2 extracellular domain
Lane 2 : Recombinant Human EPHA2 cytoplasmic domain
Loading : 20 ng
Dilution : 1:1000
WB analysis of PC-3 whole cell lysate using GTX57687 EphA2 antibody.
Loading : 40 μg
Dilution : 1:1000
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文献和实验CD66a/CD66b/CD66c/CD66d/CD66e/CD66f/CD67分子
CD66a/CD66b/CD66c/CD66d/CD66e/CD66f/CD67 分子 CD66a 常用单克隆抗体或代号: BGP- 1 主要表达细胞: G,En [M] 分子质量(kDa)和结构: gp180- 200(胆汁糖蛋白- 1)(IgSF) 功 能: 嗜同性结合,也可识别CD62E CD66b 常用单克隆抗体或代号: MF25.1;(P100,原CD67,CGM6) 主要表达
IMMUNOSCREENING OF WITH ANTIBODY PROBES
in 10mM IPTG (MW=238.31). Briefly blot the excess solution from the filters, and wrap them in cellophane. These should be moist and stored at 4°C. We typically adsorb serum with five 150 mm Petri dishes of lysed E. coli to reduce anti-E. coli antibody
Immunization Protocol for Monoclonal Antibody Production
s.c. 35 Boost #3 IFA i.v. 42 Bleed and test for antibody 45 Boost #4 IFA i.v. 66+ 3-4 week rest
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