- ￥6300
- GeneTex
- 美国
- GTX60874
- 2025年07月12日
- WB, ICC/IF, ELISA, ChIP assay
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14)
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60874
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, ELISA, ChIP assay
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H3K14ac (Acetyl Lys14)
- 抗体英文名：
Histone H3K14ac (Acetyl Lys14) antibody [RM130]
- 抗体名：
Histone H3K14ac (Acetyl Lys14) 抗体 [RM130]
- 规格：
100 μg
ChIP analysis of HeLa cells using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg
WB analysis of acid extracts from HeLa cellsuntreated (-) or treated with sodium butyrate (+) using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130].
Dilution : 0.5μg/ml
The GTX60874 reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.
ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130].
Red : Primary antibody
Green : Actin
The binding specificity of RM130 to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.
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文献和实验in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
and structural analysis of 14-3-3 proteins.Jones DH; Martin H; Madrazo J; Robinson KA; Nielsen P; Roseboom PH; Patel Y; Howell SA; Aitken A Laboratory of Protein Structure, National Institute for Medical Research, London, U.K. J Mol Biol 245: 375-84
Introduction of human 14-3-3 proteins
and structural analysis of 14-3-3 proteins.Jones DH; Martin H; Madrazo J; Robinson KA; Nielsen P; Roseboom PH; Patel Y; Howell SA; Aitken ALaboratory of Protein Structure,National Institute for Medical Research,London,U.K.J Mol Biol 245: 375-84 (1995)The 14
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