PS-CONTAINER,STERILE,330ML 68,

Human Peripheral Blood Mononuclear Cell Preparation

from the final cell sample. The isolated cells can be used in chemotaxis assays    Procedure Hint #1 ) from the bag into a 125 ml conical polypropylene centrifuge tube and dilute to 90 ml with sterile endotoxin-free PBS. 2. Add 15 ml of sterile

Hints and precautions for the care, feeding and breeding of Neurospora

, above 30°C. We routinely make crosses on SC slants in 18 X 150 mm tubes, using foam or cotton plugs. Push-on metal or plastic caps should be avoided with cross tubes because they result in more rapid water loss and are more prone to contamination

Transformation of E. coli by Electroporation

for electroporation. 1. Use a fresh colony of DH5α (or other appropriate host strain) to inoculate 5 ml of SOB (without magnesium) medium in a 50 ml sterile conical tube. Grow cells with vigorous aeration overnight at 37EC. 2. Dilute 2.5 ml of cells into 250