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文献和实验Two-hybrid analysis of genetic
into a sterile 150 mm plate,or into the sterile top from a box of 200 µl pipets,and use the 96-prong device,sterilized in ethanol and flame,to transfer the culture to -w Glu plates.On these plates,all 96 positions will contain the same prey strain. 4.Follow
Gene Knockout Techniques(1) The Knockout Process
it for even illumination of the correct well. Carefully remove the empty plate without disturbing the support, put the lamp blocking slide in place, and turn the filter set back to UV. After the worms have soaked in TMP for one hour, remove the plate lid, carefully place
, so you will only use the four dilutions that you have produced. Using four separate petri plates containing 15 ml. of nutrient agar each and four separate sterile pipettes, transfer 1.0 ml of each dilution broth suspension onto the surface of a petri plate. Carefully
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