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文献和实验Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
. Embryos may be implanted in both uterine horns or unilaterally. The transfer pipette can be loaded a number of different ways; the following is one popular method. Take up an amount of Hepes buffered medium in the tip of the transfer pipette, then make
the LCM procedure.TIP: The number of cells needed to successfully perform the assay varies depending on the quality and processing conditions of the tissue samples. One thousand cells is recommended as a good starting point.Suspend approximately 1000
! 14. Tip the slide up and allow stain to run off the slide and onto the paper towel. 15. Mount by placing a drop of PBS/glycerol on slide, add large coverslip and seal edges with clear nail polish. 16. Examine slide for presence of stained
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