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文献和实验Filter Binding Assay for EBNA-1
to binding temperature by addition of ice cold Binding Buffer to a final volume of 1000ul, incubation on ice until loading on filterPass pre-incubation through filter by increased pressure supplied by syringe above filter. Remove syringe from filter apparatus
Single Primer ("Semi-Random") P
we wish. Isolate DNA. We generally core the band with a yellow tip, and soak it overnight at 4° in a small amount of TE or H2. The supernatant provides the template for reamplification. It is also useful to reamplify the unfractionated products
Transformation of Electrocompetent E. coli with Blue/White selection
(L.B.) Bacteria culture tubes P1000, P200, P20 Pipetman and sterile tips Sterile Pasteur pipets and bulb. 37ºC incubator and rotating wheel L.B./Amp bacterial culture plates (or other suitable selection media) X-gal 20 mg/mL
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