201ul Tecan-Style Liquid Level

Sterile Technique

before leaving the lab is to wash your hands thoroughly. These are guidelines. You may find a set of techniques that best suite your working style. This is fine as long as you adhere to the basic concepts of good sterile technique.  

Yeast sporulation (liquid)

Yeast sporulation (liquid) 1. Grow a saturated yeast culture in YEPD. 2. Take 0.2 ml and put into 5 ml sterile water. Spin 3-5'. 3. Pour off water; resuspend cells in 5 ml 0.3% KOAc + 20 µg/ml Adenine + any required amino acids

Sterile Technique 无菌操作（细菌培养)

Good sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein expression techniques. Sterile technique refers to procedures by which cultures may be manipulated