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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
antibiotic. After incubating at 37 degrees C for 8-10 hours with 250 rpm shaking, the culture is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
. Embryos may be implanted in both uterine horns or unilaterally. The transfer pipette can be loaded a number of different ways; the following is one popular method. Take up an amount of Hepes buffered medium in the tip of the transfer pipette, then make
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