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文献和实验volume of about 0.5 ml of fixative. Use a pasteur pipette to transfer the cells onto clean, subbed slides and allow to air dry. Prepare slides for autoradiographic analysis as in Exercise 11.4 . Coat with nuclear track emulsion and expose for one week
Embryo Dissection and Micromanipulation Tools
or coarse forceps can be used to make adjustments to the angles of fine forceps, but any excessive bend will break the tips. Hair Loops Loops of human hair mounted in beeswax at the tip of a cut-off Pasteur pipette are used for steadying
Preparation and purification of biotin-labeled probe for ISH
with siliconized glass wool. Fll the syringe to the top with Sephadex G-50 using a sterilized pasteur pipette. Place the Sephadex-filled syringe into a 15 ml Corex tube and centrifuge at 1,500 rpm for 4 min to pack down the Sephadex.
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