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文献和实验off when you're done with the 96-pack and have entered all the data for those strains in the database. Storage in 2-ml tubes: Patch strain out on a YPD-G418 plate. Color-code the caps of the Sarstedt tubes with a VWR pen or equivalent --
Hints and precautions for the care, feeding and breeding of Neurospora
magnification. Illumination from above is best for isolating ascospores, with a white plate on the microscope stage to maximize contrast of the viable black spores and distinguish them from spores that are unripe or defective. Lighting from below using
Western Blot 1、Run protein gel.Set up blot:2、Prepare two tupperware containers, one with dH2Oand one with 1/2X Transfer Buffer (TB).3、Remove one glass plate from the gel by twisting a spacer between the plates. To remove the other plate, place
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