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文献和实验appropriate for the sample, and, of course, costs when considering the use of kits. 2. 这些浓缩方法你知道吗? Because it is usually more convenient to store antibodies or other proteins at >1 mg/ml, it may be necessary to concentrate the solution. In addition
miRNA microarrays(McManus Lab)(芯片)
Diamond-tipped glass etching pen (VWR 52865-005) StrataLinker for UV cross-linking Protocol a) Pour 100 ml 0.5x SSC into hydration tray and warm on slide warmer. Set the warmer to 37℃ b) Pre-heat a heating block at max (>100
°C). Add 2.5 µl of 6X DNA loading buffer. Prepare the solution for the polyacrylamide gel. Mix 15 ml of 40% acrylamide/bisacrylamide stock solution, 12 ml of 5X TBE buffer, and 32.5 ml of H2O. Add 50 µl of TEMED and 500 µl of freshly prepared 10
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