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文献和实验96-well RNA In Situ Hybridization Protocol
3 pH 10.2 to each well. 7. Incubate at 60 degrees C for 20 min. (This incubation time works best for 1-3 kb templates). 8. Place plate on ice, then quickly add 50ul 7.5M NH4 acetate to each well. 9. Transfer samples to 1.5ml eppendorf
that were previously immunized and then boosted IV 72 hours prior to hybridization. Media: Auto-Pow (powdered MEM from Flow Labs) with sodium bicarbonate plus non-essential amino acids, penicillin-streptomycin, L-glutamine and HT. For the serum-containing media
. During the nebulization process, unavoidable leaks are minimized by securely tightening the lid for nebulizer chamber and sealing the larger hole in the top piece with a plastic cap. To prepare for fragment end-repair, the nebulized DNA typically is divided
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