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文献和实验. Carefully open the tubes and place on the Biomek tablet.. 4. The Biomek will distribute two 250 ul aliquots of viral supernatant per sample into the wells of a 96-well flat-bottomed microtiter plate (Dynatech). The Biomek then will add 50 ul of 20% PEG
. 37. Centrifuge the tube at 16,000g for 5 min at 4°C and remove the entire supernatant with a drawn-out glass pipette. 38. Air-dry the pellet until it starts to turn from white to clear, then dissolve it in 20 µL of DEPC-treated
microcentrifuge tubes 6‐well tissue culture treated polystyrene cell culture plate (BD Falcon, cat. no. 353224) 6‐well ultra low
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