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文献和实验Good sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein expression techniques. Sterile technique refers to procedures by which cultures may be manipulated
media. 6 3. Insert the tip of a sterile pipette into the stock bottle and remove 10 ml of media. Transfer the media to the tissue culture flask. 4. Open the top of the suspension culture and use a sterile 1.0 ml transfer pipette to remove a 1.0 ml
containment level Centrifuge CO2 Incubator set at 37oC Microscope (uv Epi-Fluorescent.) 35mm plastic tissue culture dishes (Prod. No. C6296) Multidish 24 well (Prod. No. M9655) Cell scraper Microscope slides and 22mm cover slips Aluminum foil
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