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文献和实验containment level Centrifuge CO2 Incubator set at 37oC Microscope (uv Epi-Fluorescent.) 35mm plastic tissue culture dishes (Prod. No. C6296) Multidish 24 well (Prod. No. M9655) Cell scraper Microscope slides and 22mm cover slips Aluminum foil
Cell Suspension Culture of Arabidopsis
the resulting crust of seeds through a sterile tea-strainer in order to separate the individual seeds. Store the seeds in a sterile 60 mm Petri dish wrapped in Parafilm until use. 4. Lift the sterile seeds with a flamed spatula onto solid MS0 medium in screw
concentrations will be: 1 X 10 cells/ml 5 X 10 cells/ml 1 X 10 cells/ml For example: If you have 25 ml of fresh media, you will need to add 25 X 10 cells to obtain a final concentration of 1 X 10 cells/ml If your suspension culture
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