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文献和实验-inactivated)5 mg/ml gentamycin (1:100 of the lab stock)Split 2× per week 1:10. Culture in 10% CO2, 37℃ Materials Neuroprobe 96-well chemotaxis plate 5 mm pore size CAT# ChemoTx# 101-5Favorite chemokine of choice (100 mg/ml)Fixation media: 2.5% FBS
Chemotaxis Assay趋化性实验【Harvard Medical School 】
of trypan blue in one well of a 96-well plate. Take 10 m l and load onto a hemacytometer. Count the outer 4 squares and the middle square. Divide this number by 5 to get the number of cells per square. One square is equal to 1x104 cells/ml of a 1:10 dilution
Procedure Count cells. Add 10 ml of cells to 90 ml of trypan blue in one well of a 96-well plate. Take 10 ml and load onto a hemacytometer. Count the outer 4 squares and the middle square. Divide this number by 5 to get the number of cells per square
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