- ￥3500 - 4500
- 欣润生物（NEWGAINBIO)
- 江苏无锡
- IH1017
- 2025年12月15日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Immortalized Human Umbilical Artery Endothelial Cells
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
永生化
- ATCC Number：
无
- 品系：
人源
- 组织来源：
脐动脉
- 相关疾病：
无
- 物种来源：
人源
- 免疫类型：
不详
- 细胞形态：
梭形
- 是否是肿瘤细胞：
否
- 器官来源：
脐动脉
- 运输方式：
常温
- 年限：
/
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化人脐动脉内皮细胞简介：
产品描述：脐带是胎儿和胎盘之间的连系结构。形状如绳索，表面光滑透明，内含结缔组织和一支脐静脉，一对脐动脉。在子宫中，子宫动脉在胎盘的母体部分出的毛细血管，与胎盘的子体部胎儿毛细血管靠近，在此处母体和胎儿的血液间进行CO2和O2，代谢产物即代谢废物和营养物质的交换。脐动脉将胎儿产生的废物运送至胎盘，脐静脉将O2和营养物质从胎盘运送给胎儿。
产品货号：IH1017
产品类型: 原代细胞建立的永生化
传代能力: 30代左右
产品形态: 梭形
培养基：永生化人脐动脉内皮细胞专用完全培养基，产品编号：IH1017-5
支原体：呈阴性
产品培养条件：37℃，5%CO2
发货方式：常温T25方瓶运输
货期：1周左右货期
vWF抗体免疫荧光染色鉴定
Phospholipase Cε Modulates Rap1 Activity and the Endothelial BarrierPhospholipase Cε Regulation of Endothelial Rap1
The phosphoinositide-specific phospholipase C, PLCε, is a unique signaling protein with known roles in regulating cardiac myocyte growth, astrocyte inflammatory signaling, and tumor formation. PLCε is also expressed in endothelial cells, however its role in endothelial regulation is not fully established. We show that endothelial cells of multiple origins, including human pulmonary artery (HPAEC), human umbilical vein (HUVEC), and immortalized brain microvascular (hCMEC/D3) endothelial cells, express PLCε. Knockdown of PLCε in arterial endothelial monolayers decreased the effectiveness of the endothelial barrier. Concomitantly, RhoA activity and stress fiber formation were increased. PLCε-deficient arterial endothelial cells also exhibited decreased Rap1-GTP levels, which could be restored by activation of the Rap1 GEF, Epac, to rescue the increase in monolayer leak. Reintroduction of PLCε rescued monolayer leak with both the CDC25 GEF domain and the lipase domain of PLCε required to fully activate Rap1 and to rescue endothelial barrier function. Finally, we demonstrate that
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
转染等可以在一个简单的过程内完成。3.流通系统应用芯片这个平台，可以设计需要多个流体驱动的光学研究装置。通过两个液槽，可以很容易地将通道充满，或者将通道和泵连接。这种装置可以应用于只能获得少量细胞的时候，如主细胞株和 干细胞 分析。4.人工毛细管可以优化芯片的几何形状便应用于其他方面，如血管系统的模拟。通道表面提供了一个研究血液成分和上皮细胞脉管壁之间的相互作用的模型。在利用ibidi GmbHR 研究中， 人脐静脉血管内皮细胞在静态灌注的通道中培养，对通道表面处理使之功能化。HUVECs细胞的形态学和生长
：取出皮块，用血管钳或镊子将表皮与真皮层分开。5、取出表皮，剪成更小的块后，置 0.25%胰酶中，37℃，30—60分钟。6、反复吹打，制成悬液。7、培养：用 80目不锈钢纱网滤过后，低速离心，吸去上清。8、直接加入培养基（Eagle加 20%小牛血清）制成细胞悬液，接种入培养瓶，CO2温箱培养。内皮细胞培养 内皮细胞易于从大血管分离培养成单层细胞，对于研究内皮细胞再生、肿瘤促血管生长因子（TAF）等有很大价值。 研究人内皮细胞培养以人脐带静脉灌流消化法最为简便，其法如下： 1、产后新鲜脐带
管钳或镊子将表皮与真皮层分开。5、取出表皮，剪成更小的块后，置0.25%胰酶中，37℃，30-60分钟。6、反复吹打，制成悬液。7、培养：用80目不锈钢纱网滤过后，低速离心，吸去上清。8、直接加入培养基（Eagle加20%小牛血清）制成细胞悬液，接种入培养瓶，CO2温箱培养。二、内皮细胞培养内皮细胞易于从大血管分离培养成单层细胞，对于研究内皮细胞再生、肿瘤促血管生长因子（TAF）等有很大价值。研究人内皮细胞培养以人脐带静脉灌流消化法最为简便，其法如下：1、产后新鲜脐带，无菌剪取10―15厘米长一段，如不
技术资料