- ￥3500 - 4500
- 欣润生物（NEWGAINBIO)
- 江苏无锡
- IH1017
- 2026年05月29日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Immortalized Human Umbilical Artery Endothelial Cells
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
永生化
- ATCC Number：
无
- 品系：
人源
- 组织来源：
脐动脉
- 相关疾病：
无
- 物种来源：
人源
- 免疫类型：
不详
- 细胞形态：
梭形
- 是否是肿瘤细胞：
否
- 器官来源：
脐动脉
- 运输方式：
常温
- 年限：
/
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化人脐动脉内皮细胞简介：
产品描述：脐带是胎儿和胎盘之间的连系结构。形状如绳索，表面光滑透明，内含结缔组织和一支脐静脉，一对脐动脉。在子宫中，子宫动脉在胎盘的母体部分出的毛细血管，与胎盘的子体部胎儿毛细血管靠近，在此处母体和胎儿的血液间进行CO2和O2，代谢产物即代谢废物和营养物质的交换。脐动脉将胎儿产生的废物运送至胎盘，脐静脉将O2和营养物质从胎盘运送给胎儿。
产品货号：IH1017
产品类型: 原代细胞建立的永生化
传代能力: 30代左右
产品形态: 梭形
培养基：永生化人脐动脉内皮细胞专用完全培养基，产品编号：IH1017-5
支原体：呈阴性
产品培养条件：37℃，5%CO2
发货方式：常温T25方瓶运输
货期：1周左右货期
vWF抗体免疫荧光染色鉴定
Phospholipase Cε Modulates Rap1 Activity and the Endothelial BarrierPhospholipase Cε Regulation of Endothelial Rap1
The phosphoinositide-specific phospholipase C, PLCε, is a unique signaling protein with known roles in regulating cardiac myocyte growth, astrocyte inflammatory signaling, and tumor formation. PLCε is also expressed in endothelial cells, however its role in endothelial regulation is not fully established. We show that endothelial cells of multiple origins, including human pulmonary artery (HPAEC), human umbilical vein (HUVEC), and immortalized brain microvascular (hCMEC/D3) endothelial cells, express PLCε. Knockdown of PLCε in arterial endothelial monolayers decreased the effectiveness of the endothelial barrier. Concomitantly, RhoA activity and stress fiber formation were increased. PLCε-deficient arterial endothelial cells also exhibited decreased Rap1-GTP levels, which could be restored by activation of the Rap1 GEF, Epac, to rescue the increase in monolayer leak. Reintroduction of PLCε rescued monolayer leak with both the CDC25 GEF domain and the lipase domain of PLCε required to fully activate Rap1 and to rescue endothelial barrier function. Finally, we demonstrate that
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
1，主要表达于血管内皮细胞。hFLT1启动子是目前比较常用的血管内皮细胞特异性启动子。 经验分享 高脂饮食会诱导冠状动脉内皮细胞（CAECs）中信号通路失调，进而导致血管张力调节异常、组织灌注改变，并增加冠状动脉疾病的风险。江南大学无锡医学院的研究团队发表了关于调节冠状动脉血管张力的研究成果。研究人员利用和元构建的携带hFLT1启动子的AAV产品，在野生型小鼠血管内皮细胞中特异性过表达KCa3.1和KCa3.1-AR
glutamine-pyruvate-phenol-red-no-hepes.html DMEM (HY-K3001) 是广泛使用的基础培养基，适于培养多种哺乳动物细胞，包括 Hela、293、Cos-7、PC-12 等细胞系，以及原代成纤维细胞、神经元、神经胶质细胞、人脐带静脉内皮细胞、平滑肌细胞等。 DMEM/F-12 (1:1) 培养基，含 L-谷氨酰胺、酚红和 HEPES www.medchemexpress.cn/inhibitor-kit/dmem-f-12-1-1-l-glutamine-phenol-red
转染等可以在一个简单的过程内完成。3.流通系统应用芯片这个平台，可以设计需要多个流体驱动的光学研究装置。通过两个液槽，可以很容易地将通道充满，或者将通道和泵连接。这种装置可以应用于只能获得少量细胞的时候，如主细胞株和 干细胞 分析。4.人工毛细管可以优化芯片的几何形状便应用于其他方面，如血管系统的模拟。通道表面提供了一个研究血液成分和上皮细胞脉管壁之间的相互作用的模型。在利用ibidi GmbHR 研究中， 人脐静脉血管内皮细胞在静态灌注的通道中培养，对通道表面处理使之功能化。HUVECs细胞的形态学和生长
技术资料
