产品说明: Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Source
Engelbreth-Holm-Swarm Mouse Tumor
Quality
-Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
-Extensive PCR testing is performed to screen for a number of pathogens
-Tested and found negative for bacteria, fungi, and mycoplasma
-Protein concentrations determined by Lowry method
-Endotoxin units measured by Limulus Amoebocyte Lysate assay
-Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
-Biological activity is determined for each lot using a neurite outgrowth assay.
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws.
Application
Cell Growth and Differentiation
Metabolism/Toxicology Studies
Invasion Assays
in vitro and in vivo Angiogenesis Assays
in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice