ATCC 31-3-4S细胞

产品名称：ATCC 31-3-4S细胞
产品货号： HB-77
Species：mouse/mouse
Source/Application： hybridoma
Morphology： lymphoblast
Growth Mode： suspension
启动冷冻培养物
1、 准备一个培养容器，使其包含产品表上列出的推荐体积的适当培养基，并对温度和pH（CO2）进行调节。
2、 在37°C的水浴中或该细胞系的正常生长温度下，通过轻轻搅拌融化小瓶。融化应该很快，大约2分钟或直到冰晶融化。
3、 将小瓶从水浴中取出，并通过浸入或喷洒70%的乙醇对其进行消毒。在层流罩中遵循严格的无菌条件进行所有进一步的操作。
4、 拧下小瓶顶部，将内容物转移到装有9 mL推荐培养基的无菌离心管中。通过温和离心（以125×g离心10分钟）去除冷冻保护剂（DMSO）。丢弃上清液，并将细胞重悬于1或2 mL完全生长培养基中。将细胞悬浮液转移到含有完整生长培养基的培养容器中，并通过轻轻摇动充分混合。
5、 24小时后检查细胞培养物，并根据需要进行传代培养。
Initiating frozen cultures
1、Prepare a culture vessel so that it contains the recommended volume of the appropriate culture medium as listed on the Product Sheet, equilibrated for temperature and pH (CO2).
2、Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted.
3、Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol. Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations.
4、Unscrew the top of the vial and transfer the contents to a sterile centrifuge tube containing 9 mL of the recommended medium. Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10 minutes at 125 × g). Discard the supernatant, and resuspend the cells in 1 or 2 mL of complete growth medium. Transfer the cell suspension into the culture vessel containing the complete growth medium and mix thoroughly by gentle rocking.
5、Examine the cell cultures after 24 hours and subculture as needed.