- ￥6200
- GeneTex
- 美国
- GTX18143
- 2025年07月07日
- WB, ICC/IF, IHC-P, IP, ELISA
- Mouse
- Human, Mouse, Rat, Bovine
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Bovine Rhodopsin
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Biotin
- 适应物种：
Human, Mouse, Rat, Bovine
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Bovine
- 目录编号：
GTX18143
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IP, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
- 抗体英文名：
Rhodopsin antibody [1D4] (Biotin)
- 抗体名：
Rhodopsin 抗体 [1D4] (Biotin)
- 规格：
100 μg
WB analysis of human cell lysates using GTX18143 Rhodopsin antibody [1D4] (Biotin).
Loading : 15 μg
Dilution : 1:1000
IHC-P analysis mouse backskin tissue using GTX18143 Rhodopsin antibody [1D4] (Biotin).
Fixation : Bouin’s Fixative
Dilution : 1:100
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文献和实验Tse LV et al., Proc Natl Acad Sci U S A 2022 (PMID:35476513)
：免疫亲和层析；SEC：排阻层析。 参考文献： 1. Hodges, R.S., et al. 1988. Antigen-antibody interaction. J Biol Chem 263: 11768-11775.2. Oprian, D.D., et al. 1987. Expression of a synthetic bovine rhodopsin gene in monkey kidney cells. Proc Natl Acad Sci USA 84: 8874
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
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