In Vitro: Pozelimab binds with human C5 and cynomolgus monkey C5 with Kd values of 262 pM and 8.55 nM, respectively at 37˚C, pH7.4.Pozelimab blocks classic pathway (CP)-mediated hemolysis in normal human and cynomolgus monkey serum with IC50 values of 2.8 and 20 nM, respectively at 37˚C, pH7.4.Pozelimab blocks alternative pathway (AP)-mediated hemolysis in normal human and cynomolgus monkey serum with IC50 values of 26 and 8.9 nM, respectively at 37˚C, pH7.4.Pozelimab binds to human C5, cynomolgus monkey C5, human R885C C5 and human C5b,6 with Kd values of 0.189, 2.73, 0.422, 0.465 and 0.137 nM respectively at 25˚C, pH7.4.
In Vivo: Pozelimab (15 mg/kg; s.c., once) has a prolonged pharmacokinetic (PK) with a half-life of approximately 13 days in male and female C5hu/hu mice.