AapCas12b is an RNA-mediated endonuclease that binds to and cleaves specific sites of target DNA under the guidance of single-stranded guide RNA.
Cat. No.: AapCas12b-100 (for 100pmol)
Cat. No.: AapCas12b-1k (for 1000pmol)
Description
When AapCas12b recognizes and cleaves the target double-stranded DNA under the guidance of guide RNA, its "accessory cleavage" activity is activated, which can efficiently cleave non-specific single-stranded DNA (ssDNA) in the reaction system. By designing ssDNA probes with fluorescent groups or other markers at both ends, CRISPR/Cas12b can detect DNA templates and amplify the signal, to realize the detection of the target. The system has high sensitivity and strong specificity.