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文献和实验Hormone-Sensitive Lipase and Neutral Cholesteryl Ester Lipase
in human macrophages is more questionable, since attempts to demonstrate HSL in these cells have so far been unsuccessful (9 ). It is speculated that HSL in macrophages functions as a cholesteryl ester lipase in hydrolyzing stored cholesteryl esters
Human Mast Cell Proteases: Activity Assays Using Thiobenzyl Ester Substrates
also are found in some human mast cells. Methods for measuring the activities of these enzymes using sensitive synthetic peptide thiobenzyl ester substrates are described in this chapter. Using a visible plate reader with kinetic software femtomole quantities
Triglyceride Lipase Assays Based on a Novel Fluorogenic Alkyldiacyl Glycerol Substrate
is characterized by an α/β hydrolase fold (1 ). Most lipases utilize the catalytic triad Ser-His-Asp, with the serine residue acting as the nucleophile in the initial step of carboxyl ester hydrolysis; this active site serine is contained within the Gly-Xaa-Ser
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