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文献和实验Recombinant Cell Lines Stably Expressing Functional Ion Channels
acetylcholine receptor alpha7 (nAChRα7) in a host cell, we show a way to construct and use such a stable cell-based expression system for electrophysiological assays.
Detection of Soluble CR3 (CD11b/CD18) by Time-Resolved Immunofluorometry
In the cell membrane complement receptor 3 (CR3) consists of one alpha chain (CD11b) and one beta chain (CD18). CR3 participates in many immunological processes, especially those involving cell migration, adhesion, and phagocytosis
cells. We present here (i) a detailed protocol for the production and purification of soluble recombinant NK cell receptors tagged with human IgG1-Fc (thus termed receptor-Fc chimera or receptor-Ig fusion protein) and (ii) a protocol for cell staining
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