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文献和实验Generation of Recombinant Adeno-Associated Virus
open reading frame (ORF) encodes four Rep proteins, Rep78, Rep68, Rep52, and Rep40. The right ORF encodes three Cap proteins, VP1, VP2, and VP3.
In-Frame Cloning of Synthetic Genes Using PCR Inserts
any sequence or length can be built using the in-frame cloning method. Genes are assembled such that open reading frames are maintained by linking DNA fragments through the use of six basepair blunt-end restriction sites. Each cloning step uses an anchored
Materials: Chromosome medium (one bottle per student)--- KaryoMAX Peripheral Blood Karyotyping Medium #41675-018 These bottles usually come in groups of 10 (each bottle conmtaining 5 mL of media). Colcemid® solution (10 mL
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