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文献和实验Tumor Necrosis Factor- Quantification and Expression by In Situ Hybridization
The pleiotropic cytokine, tumor necrosis factor-α (TNF-α), has been shown to affect not only pathological and inflammatory conditions, but also plays a seminal role in the maintenance of physiological homeostasis. Due to these diverse
Production and Characterization of Recombinant Human and Murine TNF
Here we describe the methods for the expression of human and murine soluble tumor necrosis factor (hTNF and mTNF) in Escherichia coli cells and for their extraction, purification, and characterization. The expression and purification procedure
Induction of Tumor Cell Apoptosis by TRAIL Gene Therapy
Members of the tumor necrosis factor (TNF) superfamily influence a variety of immunological functions, including cellular activation, proliferation, and death, upon interaction with a corresponding superfamily of receptors
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