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文献和实验The discovery of hepatitis B virus (HBV) four decades ago initiated the progressive increase in knowledge of an important hepatotropic infectious agent that culminated in the design of the first successful recombinant vaccine for a human
Two dimensional nano-high-performance liquid chromatography (nanoHPLC) coupled directly to a high-resolution tandem mass spectrometer (2D-nLC-MS/MS) is an excellent method for analyzing very complex peptide mixtures, especially
with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles
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