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文献和实验Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
reading frames, an adaptation that reveals the sites of translation initiation by pre?treating cells with harringtonine to immobilize initiating ribosomes is described. The protocol described requires 5 to 7 days to generate a completed ribosome profiling
Anti-c-erb-B-2 Ribozyme for Breast Cancer
growth factor receptor (EGFR) (1 ,2 ) The putative ligand for c-erb -B-2 is thought to be gp30; however, its role in this process remains to be elucidated (3 ) Although EGFR can transform mouse NIH3T3 cells only in the presence of ligand and receptor
Transgenic Mouse and Transgenic Rat Outline
such as lacZ or a transcription factor). DNA is prepared and microinjected into fertilized mouse eggs. Potentially transgenic mice or rats are born. Transgenic founders are identified, usually by a simple PCR assay, and bred to produce offspring for analysis
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