In Vitro: Minalrestat (100 μM) decreased intracellular sorbitol without affecting intracellular glucose in primary cultured rat mesangial cells. Minalrestat (100 μM, 48 h) causes accumulation of PKC-α and -β2 in primary cultured rat mesangial cells.
In Vivo: Minalrestat (Oral gavage, 10 mg/kg/day, for 30 days) corrects the decreased microvascular reactivity in diabetic rats. Minalrestat (Oral gavage, 10 mg/kg) restores the reduced number of leukocytes adhered and migrated leukocytes in postcapillary venules in diabetic rats.