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- 2026年07月22日
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- 文献和实验
- 技术资料
- 抗体名：
CD77 Antibody (PE) 抗体
- 抗体英文名：
CD77 Antibody (PE)
- 靶点：
CD77
- 应用范围：
FC
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1088485
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
PE
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Lewis Rat IgM
- 免疫原：
详询
- 规格：
100 T
产品子类型: Primary Antibody
描述: CD77 Antibody (PE)
克隆号: 38.13
别名: globotriaosylceramide Gb3, Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer, P blood group
应用说明: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
免疫原: Daudi cell line (Burkitt´s lymphoma)
防腐剂: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
纯化方法: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
特异性: The rat monoclonal antibody 38.13 recognizes CD77 (globotriaosylceramide Gb3), a neutral glycosphingolipid expressed mainly on the surface of B cell populations, such as germinal center B cells and Burkitt´s lymphoma cells.
研究领域: Immunology & Inflammation
Entrez 基因 ID: 35
保存条件: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
备注: For research use only.
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文献和实验Western Blotting with Horseradish Pe
7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED IN ALL STEPS
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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