- ￥1350
- Solarbio
- K900031P-50ul
- 北京
- 2025年12月31日
- WB
- Rabbit
- Arabidopsis thaliana
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
PIF1
- 应用范围：
WB
- 宿主：
Rabbit
- 适应物种：
Arabidopsis thaliana
- 保质期：
一年
- 供应商：
北京索莱宝科技有限公司
- 克隆性：
Polyclonal Antibody
- 保存条件：
Store at -20°C. Avoid freeze / thaw cycles.
- 形态：
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
- 亚型：
IgG
- 免疫原：
KLH-conjugated synthetic peptide of PIF1 in Arabidopsis thaliana AT2G20180
- 规格：
50ul
产品简介
| 别名 | BHLH15;EN101;PIL5;bHLH transcription factor bHLH015 |
| 英文名称 | Anti-PIF1 polyclonal Antibody for Plant |
| 靶标 | PIF1 |
| 交叉反应 | Arabidopsis thaliana |
| 应用 | WB |
| 亚细胞定位 | Nucleus |
| 背景资料 | Transcription activator. Regulates negatively chlorophyll biosynthesis and seed germination in the dark, and lightinduced degradation of PIF1 relieves this negative regulation to promote photomorphogenesis. Binds to the G-box motif (5'-CACGTG-3') found in many light-regulated promoters. Promotes the expression of SOM, and thus modulates responses to abscisic acid (ABA) and gibberellic acid (GA). |
| 宿主 | Rabbit |
| 克隆类型 | Polyclonal Antibody |
| 蛋白分子量 | 53 kDa |
| 实际分子量 | 53 kDa |
| 免疫原 | KLH-conjugated synthetic peptide of PIF1 in Arabidopsis thaliana AT2G20180 |
| 亚型 | IgG |
| 纯化方法 | Octanoic acid-ammonium sulfate precipitation |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| Gene ID | 816538 |
| Swiss Prot | Q8GZM7 |
| 稀釋比例 | WB 1:1000-3000. |
| 单位 | 支 |
| 规格 | 50ul 100ul |
| 单位 | 支 |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图
Western blot analysis with PIF1 antibody diluted at 1:2000; Lane: Arabidopsis thaliana.
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文献和实验Generation of Plant Protein Microarrays and Investigation of Antigen-Antibody Interactions
we describe a method to generate plant protein microarrays and to use them for characterizing monoclonal antibodies or polyclonal sera with regard to their specificity and cross-reactivity. The method starts with characterized E. coli cDNA expression clones encoding
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
suspended in PBS. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600
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