- ￥2454
- CUSABIO
- 武汉
- CSB-PA129081
- 2025年07月16日
- ELISA, WB
- Rabbit
- Human, Mouse, Rat, Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of Serine 50(D-V-S(p)-P-F) derived from Human PTP1B.
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
-20°C
- 克隆性：
多克隆
- 标记物：
Non-conjugated
- 适应物种：
Human, Mouse, Rat, Monkey
- 保质期：
12个月
- 抗原来源：
Human
- 目录编号：
CSB-PA129081
- 级别：
多克隆抗体
- 库存：
充足
- 供应商：
武汉益普生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
ELISA, WB
- 靶点：
PTPN1
- 抗体英文名：
Phospho-PTPN1 (Ser50) Antibody
- 规格：
100μl
| 产品名称 | Phospho-PTPN1 (Ser50) Antibody |
| 货号 | CSB-PA129081 |
| 别名 | body; Non receptor tyrosine phosphatase 1 antibody; Protein phosphotyrosylphosphatase 1B antibody; Protein tyrosine phosphatase 1B antibody; Protein tyrosine phosphatase non receptor type 1 antibody; Protein tyrosine phosphatase placental antibody; Protein-tyrosine phosphatase 1B antibody; PTN1_HUMAN antibody; PTP 1B antibody; PTP-1B antibody; PTPN 1 antibody; PTPN1 antibody; Tyrosine protein phosphatase non receptor type 1 antibody; Tyrosine-protein phosphatase non-receptor type 1 antibody |
| 抗体类型 | Polyclonal Antibody |
| 克隆类型 | Polyclonal |
| Uniprot No. | P18031 |
| 基因名 | PTPN1 |
| 免疫原种属 | Human |
| 免疫原 | Peptide sequence around phosphorylation site of Serine 50(D-V-S(p)-P-F) derived from Human PTP1B. |
| 宿主 | Rabbit |
| 反应种属 | Human, Mouse, Rat, Monkey |
| 应用范围 | ELISA, WB |
| 抗体亚型 | IgG |
| 标记方式 | Non-conjugated |
| 产品提供形式 | liquid |
| 纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugateds. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi |
| 研究领域 | Signal Transduction |
| 保存缓冲液 | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 储存条件 | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
DEAE-Sephadex A-50 提取法纯化多克隆抗体 DEAE-Sephadex A-50(简称A-50)为弱碱性阴离子交换剂，经过NaOH处理将Cl- 型转为OH- 型后，可吸附酸性蛋白。γ1球蛋白属中性蛋白，等电点为pH6.85～7.5，其余均属酸性蛋白。在溶液为pH8.0时，酸性蛋白均被A-50 吸附。从而可分离纯化IgG。 1. 批量吸附法 此法可在1天内完成提取过程，纯化前后样品体积变化不大，
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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