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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized non-phosphopeptide derived from Human MITF around the phosphorylation site of serine 180/73 (P-N-S(p)-P-M).
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
-20°C
- 克隆性:
多克隆
- 标记物:
Non-conjugated
- 适应物种:
Human, Mouse
- 保质期:
12个月
- 抗原来源:
Human
- 目录编号:
CSB-PA257242
- 级别:
多克隆抗体
- 库存:
充足
- 供应商:
武汉益普生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA, WB, IHC, IF
- 靶点:
MITF
- 抗体英文名:
MITF (Ab-180/73) Antibody
- 规格:
100μl
| 产品名称 | MITF (Ab-180/73) Antibody |
| 货号 | CSB-PA257242 |
| 别名 | BHLHE32 antibody; bHLHe32 antibody; Class E basic helix-loop-helix protein 32 antibody; CMM8 antibody; Homolog of mouse microphthalmia antibody; Mi antibody; Microphthalmia associated transcription factor antibody; Microphthalmia, mouse, homolog of antibody; Microphthalmia-associated transcription factor antibody; MITF antibody; MITF_HUMAN antibody; mitfa antibody; nacre antibody; WS2 antibody; WS2A antibody; z3A.1 antibody |
| 抗体类型 | Polyclonal Antibody |
| 克隆类型 | Polyclonal |
| Uniprot No. | O75030 |
| 基因名 | MITF |
| 免疫原种属 | Human |
| 免疫原 | Synthesized non-phosphopeptide derived from Human MITF around the phosphorylation site of serine 180/73 (P-N-S(p)-P-M). |
| 宿主 | Rabbit |
| 反应种属 | Human, Mouse |
| 应用范围 | ELISA, WB, IHC, IF |
| 抗体亚型 | IgG |
| 标记方式 | Non-conjugated |
| 产品提供形式 | liquid |
| 纯化方式 | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| 研究领域 | Epigenetics and Nuclear Signaling |
| 保存缓冲液 | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 储存条件 | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |

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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Immunoprecipitation of metabolically labeled cells
- dilute with Super E to convenient volume; add protease inhibitors, cold Met and antibody. Some antibodies work only after the antigen has been denatured (e.g. Ab 877, Ab 471): to 50 ul of sample in TMT, add 5 µl 10% SDS and incubate at 100 o C
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