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文献和实验A typical restricion digest consists of: 0.1 µg of DNA + 1 µL of enzyme + 2 µL of 10x buffer (consult Roche or NEB list) and q.s. to 20 µL with H2 O. Note that some NEB buffers also call for the addition of BSA. Digest for 2 hrs
(tetramethylbenzidine) Substrate Reagent Set: BioLegend Cat. No. 421101 TMB Stop Solution: 1M H3 PO4 or 2N H2 SO4 实验步骤 1. Coat the Plate: 1) Dilute unlabeled capture antibody to a final concentration
Measurement of Platelet Arachidonic Acid Metabolism
by cyclooxygenase to yield prostaglandin (PG) H2 . This process is aspirin-sensitive, as the drug irreversibly acetylates and inhibits cyclooxygenase, providing the basis for the old and effective antithrombotic use for aspirin, which itself underscores
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