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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
Embryonic Fibroblasts (Irradiated) 4. DMEM with GlutaMAX™-I (High Glucose) 5. KnockOut™ DMEM/F-12 6. Fetal Bovine Serum (FBS), ES Cell-Qualified 7. KnockOut™ Serum Replacement (KSR) 8. MEM Non-Essential Amino Acids (NEAA)
Procedures for In Vitro Production of Bovine Embryos
in preimplantation bovine embryos. Mol Reprod Dev 1997; 48: 25-33.Hernandez-Ledezma JJ, Sikes JD, MurpheyCN, Watson AJ, Schultz GA, Roberts RM. Expression of bovine trophoblast interferon in conceptuses derived by in vitro techniques. Biol Reprod 1992; 47: 374-380
, microRNA-mediated degradation of transcripts encoding negative regulatory proteins would also abolish PRG expression and restore IFNα responsiveness. Some recent reports showed that interferon beta (IFNβ) stimulation can boost microRNA levels in cell
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