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文献和实验Yeast transformation using lithium acetate (rapid method)
Yeast transformation using lithium acetate (rapid method) Steve Hahn Last Modified December 1997 This method works well when transforming with a plasmid and is rapid. (for highest efficiency transformation, see DMSO
Silver Acetate Autometallography (AMG)
of glass double-distilled water. (Silver acetate crystals can be dissolved by continuous stirring within about 15 min.) Citrate buffer: Dissolve 23.5 g of trisodium citrate dihydrate and 25.5 g citric acid monohydrate in 850 mL or deionized
Transformation of Lithium-Treated Yeast Cells and the Selection of Auxotrophic and Dominant Markers
through protoplast fusion is also a possibility. In contrast, the lithium acetate method is relatively quick and simple to perform. It has the advantage that the cells remain intact, and so do not fuse, and can be plated directly onto the surface of selective agar
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