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文献和实验Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
1 mM ATP ( appendix 22 ) 50 mM MnCl 2 ( appendix 22 ) 100 U/µl CircLigase I (Epicentre
. a The recipes produce 15 ml of each separating gel solution and 10 ml of sample gel, which are sufficient for a single gel with 1.5‐mm × 14‐cm × 16‐cm dimensions (Hoefer). When using Bio‐Rad equipment (1.5‐mm × 16
a change in specific activity. 3.2. Preparation of Wnt3A CM for Fractionation 1. To the filtered CM from Section 3.1 , add Triton X-100 to1% (v/v), Tris-HCl, pH 7.5, to 20 mM, and NaN 3 to 0.01%(w/v) (e.g., to 930 mL CM, add 50 mL of 20% (v/v) TritonX
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