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- 文献和实验
- 技术资料
- 英文名：
PT-K75猪鼻甲黏膜成纤维细胞
- 库存：
现货库存
- 供应商：
南京万木春
- 肿瘤类型：
/
- 细胞类型：
/
- ATCC Number：
PT-K75猪鼻甲黏膜成纤维细胞
- 品系：
PT-K75猪鼻甲黏膜成纤维细胞
- 组织来源：
鼻
- 相关疾病：
/
- 物种来源：
/
- 免疫类型：
/
- 细胞形态：
/
- 是否是肿瘤细胞：
/
- 器官来源：
/
- 运输方式：
常温/干冰
- 年限：
三代内
- 生长状态：
/
- 规格：
T25
猪鼻甲黏膜成纤维细胞PTK75
| 种属 | 猪 |
| 别称 | PTK75 |
| 组织来源 | 猪鼻黏膜 |
| 传代比例/细胞消化 | 1:2传代,消化2-3分钟 |
| 完全培养基配置 | DMEM培养基；10%胎牛血清； 1%双抗 |
| 形态 | 成纤维细胞样 |
| 生长特征 | 贴壁生长 |
| 倍增时间 | 每周 2 至 3 次 |
| 培养条件 | 气相 ：空气 ，95% ；二氧化碳 ，5%。 温度 ：37摄氏度 ，培养箱湿度为70%-80%。 |
| 冻存条件 | 冻存液 ：90%FBS ，DMSO 10%, 或使用非程序冻存液 ：官网货号JY-H040 |
| 产品使用 | 仅限于科学研究 ，不可作为动物或人类疾病的治疗产品使用。 |
Objective: Rheumatoid arthritis (RA) is an autoimmune condition that causes severe joint deformities and impaired functionality, affecting the well-being and daily life of individuals. Consequently, there is a pressing demand for identifying viable therapeutic targets for treating RA. This study aimed to explore the molecular mechanisms of osteoclast differentiation in PBMC from patients with RA through transcriptome sequencing and bioinformatics analysis.
Methods: Blood samples were collected from 20 patients with RA, including 15 females and 5 males. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation. Osteoclast differentiation was induced using a medium containing RANKL and M-CSF for 14 days, with medium changes every 2 days. After 14 days, osteoclasts were identified by TRAP staining, and multinucleated TRAP-positive cells were counted as osteoclasts. Subsequently, transcriptome sequencing was performed using the Illumina Novaseq 6000 platform, and differential expression analysis was conducted using the DESeq2 package in R. Differentially expressed genes were selected with a significance threshold of p < 0.05 and a fold change ≥ 2 (|Log2FC|≥ 1). Bioinformatics analysis was performed using R, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses.
lktastatic disease. For such patients, attempting to treat nulkrous sites of disease compounds potential risks without a clear clinical benefit and, as such, a compelling justification for performing an intervention that is unlikely to alter a patient's clinical trajectory is uncommon. However, the discovery of immune checkpoints and the developlknt of
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文献和实验lktastatic disease. For such patients, attempting to treat nulkrous sites of disease compounds potential risks without a clear clinical benefit and, as such, a compelling justification for performing an intervention that is unlikely to alter a patient's clinical trajectory is uncommon. However, the discovery of immune checkpoints and the developlknt of
技术资料