U3人膀胱癌细胞（附STR鉴定报告）、U3细胞

产品名称：U3人膀胱癌细胞、U3人膀胱癌细胞、U3人膀胱癌细胞、U3人膀胱癌细胞产品基本信息 细胞名称：U343，人神经胶质母 种属来源：人 组织来源：脑 细胞形态：上皮细胞样 生长特性：贴壁生长 培养基： 生长条件：气相：95%空气+5%二氧化碳；温度：37℃ 传代方法：1:2至1:3，每周 3次 冻存条件：无血清冻存液，液氮储存 支原体检测：无 细胞培养操作 1）复苏细胞：将含有1 mL细胞悬液的冻存管在 37℃水浴中迅速摇晃解冻，加4 mL培养基混合均匀。在1000 rpm条件下离心3 min，弃去上清液，加1-2 mL培养基后吹匀。然后将所有细胞悬液加入含适量培养基的培养瓶中培养过夜（或将细胞悬液加入10 cm皿中，加入约8 mL培养基，培养过夜）。第二天换液并检查细胞密度。 2）细胞传代：如果细胞密度达80%-90%，即可进行传代培养。 a、弃去培养上清，用不含钙、镁离子的PBS润洗细胞1-2次。 b、加1 mL消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，使消化液浸润所有细胞，弃去消化液，将培养瓶置于37℃培养箱中消化1 min，然后在显微镜下观察细胞消化情况，若细胞大部分变圆并脱落，迅速拿回操作台，轻敲几下培养瓶后加少量培养基终止消化。 c、按6-8 mL/瓶补加培养基，轻轻打匀后装入无菌离心管中，1000 rpm离心4 min，弃去上清液，补加1-2 mL培养液后吹匀。 d、将细胞悬液按1:2比例分到新的含8 mL培养基的新皿中或者瓶中，置于培养箱中培养。 3）细胞冻存：待细胞生长状态良好时，可进行细胞冻存。下面 T25 瓶为例； a、收集细胞及细胞培养液，装入无菌离心管中，1000 rpm条件下离心4 min，弃去上清液，用PBS清洗一遍，弃尽PBS，加 1 mL血清重悬细胞，进行细胞计数。 b、根据细胞数量加入无血清细胞冻存液，使细胞密度5×106~1×107/mL，轻轻混匀，每支冻存管冻存1mL细胞悬液，注意冻存管做好标识。 c、将冻存管放入-80℃冰箱，24 h后转入液氮灌储存。记录冻存管位置以便下次拿取。

  Criminal victimization is associated with an increased risk of violent offending, which can be motivated by revenge. Experiencing revenge desire could also be harmful for crime victims mental health. To limit revenges harmful effects, researchers have examined the predictors of revenge desire and attitudes. However, little is known about the predictors of revenge desire and attitudes in crime victims specifically. This scoping review aims to identify the contextual and psychosocial predictors of revenge desire and attitudes from the existing literature. Databases (PsycInfo, PsycArticles, SCOPUS, Web of Science and MEDLINE) were searched in February 2024. Papers published in English, with data pertaining to the psychosocial and contextual predictors of revenge desire and attitudes in crime victims, were included. Quantitative studies and meta-analyses were included. Qualitative studies and reviews were excluded. Risk of bias was assessed using JBIs critical appraisal tools. 3689 records were screened. 10 reports, covering 14 studies, were included. A narrative review was conducted. Four categories of predictors were identified, namely 1) offence-related predictors, 2) mental health predictors, 3) criminal justice system predictors, and 4) demographic predictors. Significant predictors included PTSD symptoms, which were correlated with higher revenge desire, and participation in restorative justice, which was found to lower crime victims desire for violent revenge in a series of randomized controlled trials. However, this review highlights the need for further research in this area. Many predictors were merely examined in a single study, so require replication. In addition, studies were largely cross-sectional, limiting conclusions about causation.