SK-MEL-5 is amelanoma cell line isolated fromskintissueobtainedfroma24-year-old,White,femalemalignant melanoma patient. This cellline isasuitable transfectionhostandalsoservesasasourceoftarget cells for the detection of melanoma-specificantibodyinpatients withthedisease.Thisisoneof a very extensive series of melanoma linesisolatedbyT.Takahashiandassociates.
形态
星状细胞样
生长特征
贴壁生长
倍增时间
每周2 至3次
抗原表达
Blood Type O;Rh+;HLA A2, A11,B40,Bw16
致瘤性
Yes, innudemice; forms malignantmelanoma
STR
Amelogenin: X CSF1PO:10,13D13S317:10,12D16S539:10,12D5S818:11,13D7S820: 9,12 THO1: 6,9 TPOX:11 vWA:14,18
培养条件
气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。
冻存条件
冻存液:90%FBS,DMSO10%, 或使用非程序冻存液:官网货号JY-H040
保藏机构
ATCC;HTB-70
产品使用
仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。
19156044901 (同微信)
themRNAlevelsof TGF-β1andTIMP-2,andtheproteinlevelsofTGF-β1,TIMP-2, andp-β-catenin/β-cateninwereobviouslyincreased (P<0.05),the correspondingindexofTAK-715group showed the opposite trends(P<0.05) ; compared with the ATR group,the axiallength,refraction,thelevelsofIL-1β,IL-6andTNF-α,themRNAlevelofMMP-2, andtheproteinlev-elsofWntandMMP-2intheATR+TAK-715groupwereobviously