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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
SK-MEL-5
- 库存:
现货库存
- 供应商:
南京万木春生物
- 肿瘤类型:
SK-MEL-5人黑色素瘤细胞
- 细胞类型:
/
- ATCC Number:
SK-MEL-5人黑色素瘤细胞
- 品系:
/
- 组织来源:
皮肤
- 相关疾病:
/
- 物种来源:
/
- 免疫类型:
/
- 细胞形态:
/
- 是否是肿瘤细胞:
SK-MEL-5人黑色素瘤细胞
- 器官来源:
/
- 运输方式:
常温/干冰
- 年限:
三代内
- 生长状态:
良好
- 规格:
T25
皮肤恶性黑色素瘤细胞SK-MEL-5
| 种属 | 人 |
| 别称 | SK-Mel-5; SK MEL 5; SK.MEL.5; SK-MEL5; SKMel-5; SKMEL-5; SKMEL5; SKMel5; SKmel5; AA-Mel |
| 组织来源 | 皮肤 |
| 疾病 | 皮肤黑色素瘤 |
| 传代比例/细胞消化 | 1:2传代,消化2-3分钟 |
| 完全培养基配置 | MEM培养基;10%胎牛血清;1%双抗 |
| 简介 | SK-MEL-5 is a melanoma cell line isolated from skin tissue obtained from a 24-year-old, White, female malignant melanoma patient. This cell line is a suitable transfection host and also serves as a source of target cells for the detection of melanoma-specific antibody in patients with the disease. This is one of a very extensive series of melanoma lines isolated by T. Takahashi and associates. |
| 形态 | 星状细胞样 |
| 生长特征 | 贴壁生长 |
| 倍增时间 | 每周 2 至 3 次 |
| 抗原表达 | Blood Type O; Rh +; HLA A2, A11, B40, Bw16 |
| 致瘤性 | Yes, in nude mice; forms malignant melanoma |
| STR | Amelogenin: X CSF1PO: 10,13 D13S317: 10,12 D16S539: 10,12 D5S818: 11,13 D7S820: 9,12 THO1: 6,9 TPOX: 11 vWA: 14,18 |
| 培养条件 | 气相:空气 ,95% ;二氧化碳 ,5%。 温度:37摄氏度 ,培养箱湿度为70%-80%。 |
| 冻存条件 | 冻存液:90%FBS ,DMSO 10%, 或使用非程序冻存液:官网货号JY-H040 |
| 保藏机构 | ATCC; HTB-70 |
| 产品使用 | 仅限于科学研究 ,不可作为动物或人类疾病的治疗产品使用。 |
Aim: The continuous update of international guidelines and enhanced availability of biological disease-modifying antirheumatic drugs (bDMARDs) and targeted synthetic DMARDs (tsDMARDs) have made a significant impact on the diagnosis and treatment of early rheumatoid arthritis (ERA). This study aims to systematically evaluate the current treatment strategies and outcomes within a large-scale cohort of patients with ERA.
Methods: Data from the Chinese Registry of Rheumatoid Arthritis (CREDIT), a large multicenter Chinese registry of RA, were collected to analyze temporal trends in clinical profiles, therapeutic strategies, and treatment outcomes among patients with ERA.
the mRNA levels of TGF- β1 and TIMP-2,and the protein levels of TGF- β1,TIMP-2,
and p- β-catenin / β-catenin were obviously increased (P<0.05) ,the corresponding index of TAK-715 group showed the opposite trends ( P<0.05) ;
compared with the ATR group,the axial length,refraction,the levels of IL-1β , IL-6 and TNF- α , the mRNA level of MMP-2,
and the protein lev- els of Wnt and MMP-2 in the ATR+TAK-715 group were obviously
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文献和实验the mRNA levels of TGF- β1 and TIMP-2,and the protein levels of TGF- β1,TIMP-2,
and p- β-catenin / β-catenin were obviously increased (P<0.05) ,the corresponding index of TAK-715 group showed the opposite trends ( P<0.05) ;
compared with the ATR group,the axial length,refraction,the levels of IL-1β , IL-6 and TNF- α , the mRNA level of MMP-2,
and the protein lev- els of Wnt and MMP-2 in the ATR+TAK-715 group were obviously
人 乳腺导管癌 1000元 BT-549 TCHu 93 人 乳腺管癌细胞 400元 T-47D TCHu 87 人 乳腺管癌细胞 400元 CCD-1095Sk GNHu15 人 乳腺浸润性导管癌旁皮肤细胞 1000元 SK-BR-3 TCHu 49 人 乳腺腺癌细胞 400元 HFL-I GNHu 5 人 胚肺成纤维细胞 450元 MRC-5 GNHu12 人 胚肺细胞 800元 WI-38 GNHu 8 人 胚肺细胞
-I(MEMα) 胚肺成纤维细胞 Chang liver 张氏肝细胞 HA 羊膜细胞 QSG-7701 肝细胞 WISH 羊膜细胞 HL-7702 肝细胞 HBL-100 整合SV40 基因的乳腺上皮细胞 CGM1 (悬浮) EB 病毒转化的B 细胞 293 人胚肾细胞 MRC-5 胚肺细胞 WI-38
的论文所使用的就是这一细胞系。 Josephine Nefkens 研究所的分子生物学家 Winand Dinjens 发现,在 13 株食管腺癌细胞系中,其中 3 株,SEG-1,BIC-1 和 SK-GT-5 被污染,这些细胞株混合有其他癌细胞。 科罗拉多大学的遗传学家 Christopher Korch 发现,在过去 15 年期间,他发表的文章中有 78 株广泛应用的细胞系被证明受到了污染。 其中有两株存在严重污染的"明星"细胞系。一个是 HEp-2 细胞系,有 5789 篇文章
技术资料






