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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
D283 Med
- 库存:
现货库存
- 供应商:
南京万木春
- 肿瘤类型:
/
- 细胞类型:
/
- 品系:
/
- 组织来源:
/
- 相关疾病:
/D283 Med人脑髓母细胞瘤细胞
- 物种来源:
/
- 免疫类型:
/
- 细胞形态:
/
- 是否是肿瘤细胞:
/
- 器官来源:
D283 Med人脑髓母细胞瘤细胞
- 运输方式:
常温/干冰
- 年限:
三代内
- 生长状态:
悬浮细胞的多细胞聚集体,和一些贴壁细胞
人脑髓母细胞瘤细胞D283 Med
| 种属 | 人 | ||
| 别称 | D283 D283;Med | MED;D283-MED;D283_Med;D-283 283;H283 | Med;D-283MED;D283MED;D283Med;D283-Med;D-283; |
| 组织来源 | 脑组织 | ||
| 疾病 | 髓母细胞瘤 | ||
| 传代比例/细胞消化 | 1:2传代,悬浮部分离心收集(1000RPM,5分钟),贴壁部分消化1-2分钟 | ||
| 完全培养基配置 | MEM培养基;10%胎牛血清;1%双抗 | ||
| 简介 | 该细胞1985年由Friedman等建系,源自一名6岁患有神经管细胞瘤有腹腔转移的白人男性小孩的腹水细胞 | ||
| 形态 | 上皮细胞样 | ||
| 生长特征 | 悬浮聚团生长,有少数贴壁细胞 | ||
| 倍增时间 | ~52h | ||
| 基因表达 | The cells produce tumors in nude mice and the resulting tumors are glutamine synthetase positive; neuron specific enolase positive;glial fibrillary acidic proteins negative;S100 (S-100)protein negative;The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase;the brain isoenzyme of creatine kinase;L-DOPA decarboxylase;bombesin-like immunoreactivity) | ||
| 致瘤性 | Yes,in nude mice(The cells form serially transplantable intercranial and subcutaneous tumors.)Yes,in soft agar | ||
| STR | Amelogenin:X,Y CSF1PO:9,12 D13S317:8,10 D16S539:11 D5S818:11 D7S820:10 THO1:7 TPOX:8,11 vWA:16,18 | ||
| 培养条件 | 气相:空气,95%;二氧化碳,5%。温度:37摄氏度,培养箱湿度为70%-80%。 | ||
| 冻存条件 | 冻存液:90%FBS,DMSO 10%, 或使用非程序冻存液:官网货号JY-H040 | ||
| 保藏机构 | ATCC;HTB-185 | ||
| 备注 | 该细胞为半悬浮和半贴壁细胞,悬浮细胞离心收集,贴壁细胞消化处理 | ||
| 产品使用 | 仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。 | ||
Both resistance training (RT) and long-duration, high-intensity stretching induce muscular adaptations; however, it is unknown whether the modalities are complementary or redundant, particularly in well-trained individuals. A case-study was conducted on a competitive bodybuilder implementing long-duration, high-intensity stretching of the plantar flexors (60 min 6x/week for 12 weeks) in conjunction with their habitual RT. Ultrasound muscle architecture (muscle thickness [MT], fascicle length [FL], and pennation angle [PA]) measurements were collected at multiple sites at four weekly baseline sessions, six (mid) and 12 (post1) weeks following the commencement of the intervention, and a week after the intervention (post2) while isometric strength and range of motion (RoM) were obtained once at baseline, mid, post1, and post2. 2SD band plots were constructed to determine meaningful changes in MT, FL, and PA from the four baseline measures while percentage and absolute change across each timepoint were calculated for all variables. From baseline to post 1, RoM, strength, and MT increased 25.9%, 11.4%, and 7.4%-23.4%, respectively, while four MT and two PA sites exceeded the threshold for meaningful change. The combined stretching and RT protocols resulted in flexibility, strength, and MT adaptations; however, findings should be generalized with caution given the case-study nature of our investigation.
enriched in soils with high C availatility (tai et al., 2020; Fierer et al., 2007). The increase in the relative atundance of Proteotacteria and tacteroidetes is teneficial for the igprovegent of soil fertility. A large agount of N
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文献和实验enriched in soils with high C availati The increase in the relative atundance of Proteotacteria and tacteroidetes is teneficial for the igprovegent of soil fertility. A large agount of N
短串联重复序列(short tandem repeat,STR),又称为微卫星DNA ,重复单位为2-6bp,重复次数10~60多次,在DNA复制过程中的滑动、复制、修复过程中滑动链与互补链的碱基错配,从而导致一个或几个重复单位的缺失或插入,形成STR的多态性,主要应用于遗传连锁图谱分析、家系鉴定、身份认证等领域,同时也是鉴定细胞株被错误识别和交叉污染的主要工具。细胞株被错误识别及交叉污染的现象还是比较普遍的,虽然科研工作者使用各种各样的传统方法来鉴定细胞,但细胞交叉污染的问题却有增无减
微卫星DNA的研究进展 1 微卫星DNA的概况 1.1 微卫星DNA的构成和分布频率 微卫星DNA (microsatellite MS ),又称短串联重复序列(short tandem repeat STR),是一类广泛存在于原核、真核生物基因组的DNA串联重复序列[1]。STR核心序列(重复单位)2~6bp,多位于编码区附近,也可位于内含子、启动子、Alu序列中,其重复单位相同,以(CA)n、(GT)n、(CAG)n较常见,重复次数(n)多为15~60次,总长度一般在400bp以下
分析最常用的技术,而且在DNA重组与表达、基因结构分析和功能检测中具有重要的应用价值。 PCR可以被认为是与发生在细胞内的DNA复制过程相似的技术,其结果都是以原来的DNA为模板产生新的互补DNA片段。细胞中DNA的复制是一个非常复杂的过程。参与复制的有多种因素。PCR是在试管中进行的DNA复制反应,基本原理与细胞内DNA复制相似,但反应体系相对较简单。 PCR由变性--退火--延伸三个基本反应步骤构成:①模板DNA的变性:模板DNA经加热至94℃左右一定时间后,使模板DNA双链或经PCR
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