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文献和实验Use of Phospholipase A2 for the Production of Lysophospholipids
for their identification. For this purpose, authentic LPL standards have to be synthesized from phosphoglycerides by PLA2 digestion in vitro. PLA2 specifically hydrolyzes the fatty acid ester linkage in the sn -2-position of phospholipids to liberate sn -2-linked fatty
Phospholipases A2 (PLA2 ) catalyze the hydrolysis of the ester bond at the sn-2 position of phospholipids to generate the lysophospholipid and free fatty acid (1 ). Several classes of PLA2 s have been identified (2 ). They include the low
density lipoproteins (HDL) to mature, spherical HDL, through the generation of a cholesteryl ester (CE)-enriched core (2 ,3 ). The enzyme has also been implicated in the reverse cholesterol transport pathway, which results in the net transport
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